Wednesday, June 25, 2014

Understanding the Ebola Virus: Now at at least 60 locations in Western Africa





  1. The main problem I see is that in this particular outbreak we have 60 locations and already 350 deaths of the 567 people now known with it there. The real danger is even one person without obvious symptoms flying to Europe, Asia, North or South America, Australia or any other island or location of any size. Then if this person doesn't immediately check into a hospital with symptoms any one who touches that person can come down with Ebola. Ebola has a 90% fatality rate. 

    So, the world might be presently looking at a Pandemic of the kind we haven't seen since the Spanish Flu Outbreak in 1918. 

    Since it killed 50 to 100 million people all over the world (2% to 3% of the total world population) it may have killed more people than World War II which was the greatest human tragedy in human history so far that we know of. So, it is possible that the Flu pandemic of 1918 killed more people than anything in recorded history on earth.

    begin quote: 

    The 1918 flu pandemic (January 1918 – December 1920) was an unusually deadly influenza pandemic, the first of the two pandemics involving H1N1 influenza virus.[1] It infected 500 million[2] people across the world, including remote Pacific islands and the Arctic, and killed 50 to 100 million of them—three to five percent of the world's population[3] —making it one of the deadliest natural disasters in human history.[2][4][5][6]

    end quote from:

    1918 flu pandemic - Wikipedia, the free encyclopedia

    en.wikipedia.org/wiki/1918_flu_pandemic
    Wikipedia
    Jump to Spanish flu research - Main article: Spanish flu research. An electron micrograph showing recreated 1918 influenza virions. Centers for Disease ...


    In point of fact more  U.S. soldiers died from the Spanish Flu  than were killed in World War I

    So, understanding more about how Ebola works if it gets loose on your continent might be helpful about now.

     

     

    Ebola virus disease - Wikipedia, the free encyclopedia

    en.wikipedia.org/wiki/Ebola_virus_disease
    Wikipedia
    Ebola virus disease (EVD) or Ebola hemorrhagic fever (EHF) is the human disease caused by ebola viruses. Symptoms start two days to three weeks after ...
  2. Ebola virus - Wikipedia, the free encyclopedia

    en.wikipedia.org/wiki/Ebola_virus
    Wikipedia
    Ebola virus (EBOV) is the most dangerous virus of the Ebolavirus genus, which causes an extremely severe disease in humans and other primates, the Ebola ...
  3. Zaire ebolavirus - Wikipedia, the free encyclopedia

    en.wikipedia.org/wiki/Zaire_ebolavirus
    Wikipedia
    Zaire ebolavirus (EBOV) is a virological taxon species included in the genus Ebolavirus, family Filoviridae, order Mononegavirales. The Zaire ebolavirus is one ...
  4. Ebolavirus - Wikipedia, the free encyclopedia

    en.wikipedia.org/wiki/Ebolavirus
    Wikipedia
    Ebolavirus is the virus responsible for Ebola virus disease, a type of hemorrhagic fever. The name Ebolavirus is derived from the Ebola River in Zaire (where ...
  5. 2014 West Africa Ebola outbreak - Wikipedia, the free ...

    en.wikipedia.org/wiki/2014_West_Africa_Ebola_outbreak
    Wikipedia
    An ongoing epidemic of the Ebola virus has spread throughout Guinea and beyond the nation's borders in West Africa. At least 567 infections and 350 deaths ...

    2014 West Africa Ebola outbreak

    From Wikipedia, the free encyclopedia
    2014 West Africa Ebola outbreak
    Guinea Sierra Leone Ebola Map April 14 2014.jpg
    Situation map of the outbreak as of 18 June 2014
    Date February 2014 – Ongoing
    Location West Africa
    (Guinea, Liberia, Sierra Leone)
    Casualties
     Guinea – 390 cases, 270 deaths
     Liberia – 51 cases, 34 deaths
     Sierra Leone — 158 cases, 58 deaths[1]
    Total mortality:
    362 deaths / 599 cases (60%)
    An ongoing epidemic of the Ebola virus has spread throughout Guinea and beyond the nation's borders in West Africa. At least 567 infections and 350 deaths have been reported.[2] Various organizations, including the US Centers for Disease Control, European Commission, and Economic Community of West African States have donated funds and personnel to help counter the outbreak. This ebola outbreak is the worst ever: the most severe both in the number of cases and dead.[3]

    Initial outbreak in Guinea

    In February 2014, the first Ebola virus outbreak registered in the region occurred in Guinea. By 23 April, the total number of suspected and confirmed cases in the Ebola Haemorrhagic Fever (EHF) outbreak had increased to 242, including 142 deaths at a case fatality rate of 58.7%.[4] Originally, the suspected cases were reported in Conakry (four cases), Guéckédougou (four), Macenta (one) and Dabola (one) prefectures. On 25 March the Ministry of Health of Guinea reported that four southeastern districts: Guekedou, Macenta, Nzerekore, and Kissidougou, were affected with an outbreak of Ebola hemorrhagic fever.[5] The following day the Pasteur Institute in Lyon, France confirmed the Ebola strain as Zaire ebolavirus.[5] However, full genetic sequencing subsequently revealed that it was a new strain of Ebolavirus.[6] One suspected case was admitted to hospital on 28 March 2014.[7] On 31 March, the US Centers for Disease Control sent a five-person team "to assist Guinea Ministry of Health (MOH) and World Health Organization (WHO) led international response to the Ebola outbreak".[5]

    Conakry, Guinea

    The outbreak spread to Guinea's capital Conakry,[5] a city of about two million inhabitants, and is a major concern according to Ibrahima Touré, Country Director of Plan Guinea NGO: "The poor living conditions and lack of water and sanitation in most districts of Conakry pose a serious risk that the epidemic escalates into a crisis. People do not think to wash their hands when they do not have enough water to drink."[8]

    Subsequent spread

    Initially Sierra Leone, Mali and Ghana identified suspected cases, but all the clinical samples on those suspected cases have tested negative for ebolavirus.[9] The overall situation with the Ebola virus infection in Guinea improved in May 2014. For several days no new alerts, cases or nosocomial transmission were reported from Liberia and from 5 out of the 6 affected prefectures in Guinea. Gueckedou was the only hotspot where community transmission and deaths were still being reported.
    However, from 23 May 2014 to 27 May 2014, three previously affected districts (Guéckédou, Macenta and Conackry), four new districts (Boffa, Télimélé, Boke and Dubrecka) and one new country (Sierra Leone) reported several new EVD clinical cases.[10]

    Monrovia, Liberia

    On June 17, 2014, it was announced that seven people in Monrovia had died of Ebola.[11]

    Response

    The national authorities of Guinea, Sierra Leone, and Liberia have activated their national emergency committees, prepared EHF response plans and carried out needs assessments.[7] The Economic Community of West African States (ECOWAS) issued a statement that called for the support of the international community in the fight against the epidemic: "The Board of Mediation and Security of ECOWAS appeals to the international community to provide support in the management of the Ebola epidemic in the region."[12] On 30 March 2014, during the 44th Summit of the heads of state and government of West Africa, ECOWAS disbursed USD $250,000 to deal with the outbreak.[13] Sierra Leone has instituted a temporary measure which includes reactivation of its Active Surveillance Protocol that will see all travellers into the country from either Guinea or Liberia subjected to strict screening to ascertain their state of health.[14]
    Senegal’s Ministry of Interior has ordered all movements of people through the southern border with Guinea to be suspended indefinitely to prevent the spread of the disease, according to a statement published on 29 March 2014 by state agency APS.[15] Since 26 March 2014, Mauritania closed all crossings along the Senegal River, the natural border between Mauritania and Senegal, except for the Rosso and Diama points of entry. From 1 April 2014, Saudi Arabia stopped issuing visas for the Muslim pilgrimage to Mecca to those from Guinea and Liberia. Moreover, Morocco reinforced medical surveillance at the Casablanca airport, a regional hub for flights from and to West Africa.[16][17][18] Conversely, the border between Guinea and Liberia remained open; Guinea's ambassador in Monrovia noted his government's belief that efforts to fight the disease directly would be more effective than closing the border.[19]
    The European Commission (EC) gave €500,000 to help contain the spread of the virus in Guinea and its neighbouring countries. The EC has also sent a health expert to Guinea to help assess the situation and liaise with the local authorities. EU Commissioner for International Cooperation, Humanitarian Aid and Crisis Response Kristalina Georgieva said: "We are deeply concerned about the spread of this virulent disease and our support will help ensure immediate health assistance to those affected by it. It's vital that we act swiftly to prevent the outbreak from spreading, particularly to neighbouring countries."[20]
    A mobile laboratory, capable of performing the molecular diagnosis of viral pathogens of risk groups 3 and 4, was deployed in Guinea by the European Mobile Laboratory consortium (http://www.emlab.eu/) as part of the WHO/GOARN outbreak response. Prior samples were analyzed at the Jean Mérieux BSL-4 Laboratory in Lyon.[21]

    Complications

    As of June 2014, the outbreak was described as "totally out of control". Difficulties faced in attempting to contain of the outbreak include the outbreak's multiple locations across country borders,[22] inadequate precautions taken by medical personnel,[23] funeral practices [24] and community resistance, including "freeing" suspected Ebola patients from isolation [25]

    Virology

    Researchers performed full-length genome sequencing and phylogenetic analysis of samples from 20 patients. Results suggest that the Zaire ebolavirus (EBOV) in Guinea is a different clade than the strains from outbreaks in the Democratic Republic of Congo and Gabon.[26]

    Economic effects

    In addition to the loss of life, the outbreak is having an economic effect. Conakry's Palm Camayenne Hotel, which is popular among businessmen and politicians, has less than a third of occupancy rate.[27] On Brussels Airlines flights between the Brussels and Conakry, there are reduced numbers of people arriving in Conakry but more people leaving Conakry.[27] Regional airline Gambia Bird delayed the start of a route to Conakry due to Senegal closing "its border with Guinea because of the outbreak".[27]
    Non-essential employees of British firm London Mining in Sierra Leone were transported out of the country in June following an outbreak of the virus. At least 50 suspected cases of Ebola with 5 deaths had been reported.[28]

    Temporal evolution

    Timeline of the oubreak, using data reported by Centers for Disease Control and Prevention.[5]

    Temporal evolution of the 2014 Ebola outbreak.

    Temporal evolution of the cases per day of the 2014 Ebola outbreak.
    Date Total cases Total deaths Guinea cases/deaths Liberia cases/deaths Sierra Leone cases/deaths
    24 June 2014 599 362 390/270 51/34 158/58
    17 June 2014 528 337 398/264 33/24 97/49
    10 June 2014 474 252 372/236 13/9 89/7
    5 June 2014 438 231 344/215 13/9 81/7
    2 June 2014 354 208 291/193 13/9 50/6
    27 May 2014 309 200 281/186 12/9 16/5
    23 May 2014 270 183 258/174 12/9
    14 May 2014 245 166 233/157 12/9
    5 May 2014 243 164 231/155 12/9
    30 April 2014 233 155 221/146 12/9
    23 April 2014 220 145 208/136 12/9
    21 April 2014 215 138 203/129 12/9
    17 April 2014 209 131 197/122 12/9
    10 April 2014 169 110 157/101 12/9
    7 April 2014 163 102 151/95 12/7
    2 April 2014 135 88 127/83 8/5
    1 April 2014 130 82 122/80 8/2
    31 March 2014 114 70 112/70 2/0
    27 March 2014 103 66 103/66 0/0
    26 March 2014 86 60 86/60 0/0
    25 March 2014 86 59 86/59 0/0

    See also

    References

    1. "Outbreak of Ebola in Guinea, Liberia, and Sierra Leone". Center s for disease cpntrol and prevention. 18 June 2014.
    2. "Epidemiological update: Outbreak of Ebola Virus Disease in West Africa". WHO AFRICA. 7 June 2014.
    3. "Chronology of Ebola Hemorrhagic Fever Outbreaks". CDC. 24 June 2014. Retrieved 25 June 2014.
    4. "Ebola outbreak in Guinea 'unprecedented' – MSF". BBC News. 31 March 2014. Retrieved 31 March 2014.
    5. "Outbreak of Ebola in Guinea and Liberia". Centers for Disease Control and Prevention.
    6. "Emergence of Zaire Ebola Virus Disease in Guinea — Preliminary Report". New England Journal of Medicine. 16 April 2014. Retrieved 3 May 2014.
    7. "Ebola Haemorrhagic Fever, Guinea (Situation as of 30 March 2014)". Afro.who.int. Retrieved 31 March 2014.
    8. Diallo, Boubacar (29 March 2014). "Ebola in Guinea: the NGO Plan Guinea-feared a worsening of the epidemic ...". Africaguinee.com. Retrieved 31 March 2014.
    9. "Ebola Haemorrhagic Fever, West Africa (Situation as of 16 April 2014)". Afro.who.int. Retrieved 16 April 2014.
    10. WHO Update 27 April 2014 "Ebola Haemorrhagic Fever, West Africa (Situation as of 27 April 2014)"]. Afro.who.int. Retrieved 27 April 2014.
    11. "Seven die in Monrovia Ebola outbreak". BBC News. 2014-06-17. Retrieved 2014-06-18.
    12. "The West Africa requires the support of the international community in the fight against Ebola". abidjan.net. 28 March 2014. Retrieved 31 March 2014.
    13. "La CEDEAO décaisse 250 000 dollars pour faire face à la fièvre Ebola" (in French). abidjan.net. 30 March 2014. Retrieved 31 March 2014.
    14. Cham, Kemo (1 April 2014). "Sierra Leone restricts movement across borders over Ebola fears". Africa Review. Retrieved 1 April 2014.
    15. "Ebola Death Toll in Guinea Rises to 70 as Senegal Closes Border". Bloomberg News. 29 March 2014. Retrieved 31 March 2014.
    16. "Fièvre Ebola : contrôle sanitaire renforcé au Maroc" (in French). Nouvelobs. 1 April 2014. Retrieved 1 April 2014.
    17. "Visas refusés aux pèlerins de Guinée et du Libéria" (in French). 20min.ch. 1 April 2014. Retrieved 1 April 2014.
    18. Ndiaye Moctar, Pape (29 March 2014). "The uncrossable river Senegal since last Tuesday". Sud Quotidien. Retrieved 31 March 2014.
    19. Kwanue, C.Y. "Guinean Gov’t Provides Ebola Updates", Liberian Observer, 2014-04-07. Accessed 2014-04-07.
    20. "Ebola in West Africa: European Union joins effort to stop spread of disease and releases €500,000 in immediate funding". Europa.eu. 19 November 2013. Retrieved 31 March 2014.
    21. "Inserm and the Institut Pasteur identify a new variant of Ebola virus in Guinea". INSERM.fr. 18 Apr 2014. Retrieved 24 Apr 2014.
    22. "Doctors Without Borders: West Africa's Ebola Outbreak is Totally Out of Control". CTVNews. 20 June 2014. Retrieved 21 June 2014.
    23. "Liberia: Unheralded Fear - Ebola Pose Risks for Liberia Health Workers". allAfrica. 19 June 2014. Retrieved 21 June 2014.
    24. "Growing Ebola Outbreak Threatens to Overwhelm Volunteers". Today Health. 8 June 2014. Retrieved 21 June 2014.
    25. "8 ebola suspects freed by relatives in Sierra Leone". Global Post. 28 May 2014. Retrieved 21 June 2014.
    26. Baize, Sylvain; Pannetier, Delphine; Oestereich, Lisa; Rieger, Toni (16 Apr 2014). "Emergence of Zaire Ebola Virus Disease in Guinea — Preliminary Report". New England Journal of Medicine. doi:10.1056/NEJMoa1404505.
    27. Adama Diarra and Misha Hussain (4 April 2014). "Mali suspects first Ebola cases as regional death toll tops 90". Reuters. Retrieved 4 April 2014.
    28. "UK employees leave Sierra Leone over Ebola threat". BBC News. 3 June 2014. Retrieved 4 June 2014.

    External links

    This page was last modified on 25 June 2014 at 14:45.
    end quote from:

    2014 West Africa Ebola outbreak - Wikipedia, the free ...

     

    And the following describes more about Ebola itself:

    Ebola virus disease

    From Wikipedia, the free encyclopedia
    "Ebola" redirects here. For other uses, see Ebola (disambiguation).
    Ebola virus disease
    Classification and external resources
    7042 lores-Ebola-Zaire-CDC Photo.jpg
    1976 photograph of two nurses standing in front of Mayinga N., a patient with Ebola virus disease; she died only a few days later due to severe internal hemorrhaging.
    ICD-10 A98.4
    ICD-9 065.8
    DiseasesDB 18043
    MedlinePlus 001339
    MeSH D019142
    Ebola virus disease (EVD) or Ebola hemorrhagic fever (EHF) is the human disease caused by ebola viruses. Symptoms start two days to three weeks after contracting the virus with a fever, throat and muscle pains, and headaches. There is then nausea, vomiting and diarrhea along with decreased functioning of the liver and kidneys. At this point some people begin to have problems with bleeding.[1]
    The disease is first acquired by a population when a person comes into contact with the blood or bodily fluids of an infected animal such as a monkey or fruit bat. Fruit bats are believed to carry and spread the disease without being affected by it. Once infected the disease may be spread from one person to another. Men who survive may be able to transmit the disease sexually for nearly two months. To make the diagnosis, typically other diseases with similar symptoms such as malaria, cholera and other viral hemorrhagic fever are excluded. The blood may then be tested for either antibodies to the virus, the viral DNA, or the virus itself to confirm the diagnosis.[1]
    Prevention involves decreasing the spread of the disease from infected monkeys and pigs to humans. This may be done by checking these animals for infection and killing and properly disposing of the bodies if the disease is discovered. Properly cooking meat and wearing protective clothing when handling meat may be helpful, as may wearing protective clothing and washing hands when around someone sick with the disease. Samples from people with the disease should be handled with an extra degree of caution.[1]
    There is no specific treatment for the virus with efforts to help people including giving the person either oral rehydration therapy or intravenous fluids. The disease has a high death rate: possibly up to 90%. It typically occurs in outbreaks in tropical regions of Sub-Saharan Africa. Between 1976, when it was first identified, and 2012, fewer than 1,000 people a year have been infected. The disease was first identified in the Sudan and the Democratic Republic of the Congo. Efforts are ongoing to develop a vaccine; however, none exists as of 2014.[1]

    Signs and symptoms

    Manifestation of Ebola begins abruptly with a sudden onset of an influenza-like stage characterized by general malaise, fever with chills, sore throat, severe headache, weakness, joint pain, muscle pain, and chest pain. [2] Respiratory tract involvement is characterized by pharyngitis with sore throat, cough, dyspnea, and hiccups. The central nervous system is affected as judged by the development of severe headaches, agitation, confusion, fatigue, depression, seizures, and sometimes coma.
    Cutaneous presentation may include: maculopapular rash, petechiae, purpura, ecchymoses, and hematomas (especially around needle injection sites). In general, development of hemorrhagic symptoms is indicative of a negative prognosis. However, contrary to popular belief, hemorrhage does not lead to hypovolemia and is not the cause of death (total blood loss is low except during labor). Instead, death occurs due to multiple organ dysfunction syndrome (MODS) due to fluid redistribution, hypotension, disseminated intravascular coagulation, and focal tissue necroses.
    The average time between contracting the infection and the onset of symptoms is 13 days, but can be as long as 25 days.[3]

    Hemorrhage

    All people infected show some extent of coagulopathy and impaired circulatory system symptomology.[4] Bleeding from mucous membranes and puncture sites is reported in 40–50% of cases,[5] while maculopapular rashes are evident in approximately 50% of cases.[4] Sources of bleeds include hematemesis, hemoptysis, melena, and aforementioned bleeding from mucous membranes (gastrointestinal tract, nose, vagina and gingiva). However diffuse bleeding (i.e. heavy) is rare; occurrence is usually exclusive to the gastrointestinal tract.[4][6]

    Causes

    Main article: Ebolavirus
    EVD is caused by five of six viruses classified in the genus Ebolavirus, family Filoviridae, order Mononegavirales: Bundibugyo virus (BDBV), Ebola virus (EBOV), Sudan virus (SUDV),Taï Forest virus (TAFV) and an as-yet unnamed Ebolavirus responsible for the 2014 West Africa Ebola outbreak.[7] The sixth virus, Reston virus (RESTV), is thought to be not disease causing for humans and therefore not discussed here.

    Transmission

    EVD is believed to occur after an ebolavirus is transmitted to a human index case via contact with an infected animal host[citation needed]. Human-to-human transmission occurs via direct contact with blood or bodily fluids from an infected person (including embalming of an infected dead person) or by contact with contaminated medical equipment such as needles[citation needed]. In the past, explosive nosocomial transmission has occurred in under-equipped African hospitals due to the reuse of needles and lack of implementation of universal precautions[citation needed]. Aerosol transmission has not been observed during natural EVD outbreaks[citation needed]. The potential for widespread EVD epidemics is considered low due to the high case-fatality rate, the rapidity of demise of patients, and the often remote areas where infections occur.[citation needed]

    Risk factors


    Bushmeat being prepared for cooking in Ghana, 2013. Human consumption of equatorial animals in Africa in the form of bushmeat has been linked to the transmission of diseases to people, including ebola.[8]
    Between 1976 and 1998, in 30,000 mammals, birds, reptiles, amphibians, and arthropods sampled from outbreak regions, no ebolavirus was detected apart from some genetic traces found in six rodents (Mus setulosus and Praomys) and one shrew (Sylvisorex ollula) collected from the Central African Republic.[9][10] Traces of EBOV were detected in the carcasses of gorillas and chimpanzees during outbreaks in 2001 and 2003, which later became the source of human infections. However, the high lethality from infection in these species makes them unlikely as a natural reservoir.[9]
    Plants, arthropods, and birds have also been considered as possible reservoirs; however, bats are considered the most likely candidate.[11] Bats were known to reside in the cotton factory in which the index cases for the 1976 and 1979 outbreaks were employed, and they have also been implicated in Marburg virus infections in 1975 and 1980.[9] Of 24 plant species and 19 vertebrate species experimentally inoculated with EBOV, only bats became infected.[12]
    The absence of clinical signs in these bats is characteristic of a reservoir species. In a 2002–2003 survey of 1,030 animals including 679 bats from Gabon and the Republic of the Congo, 13 fruit bats were found to contain EBOV RNA fragments.[13] As of 2005, three types of fruit bats (Hypsignathus monstrosus, Epomops franqueti, and Myonycteris torquata) have been identified as being in contact with EBOV. They are now suspected to represent the EBOV reservoir hosts.[14][15]
    The existence of integrated genes of filoviruses in some genomes of small rodents, insectivorous bats, shrews, tenrecs, and marsupials indicates a history of infection with filoviruses in these groups as well.[16] However, it has to be stressed that infectious ebolaviruses have not yet been isolated from any nonhuman animal.
    Bats drop partially eaten fruits and pulp, then terrestrial mammals such as gorillas and duikers feed on these fallen fruits. This chain of events forms a possible indirect means of transmission from the natural host to animal populations, which have led to research towards viral shedding in the saliva of bats. Fruit production, animal behavior, and other factors vary at different times and places that may trigger outbreaks among animal populations.[17] Transmission between natural reservoirs and humans are rare, and outbreaks are usually traceable to a single index case where an individual has handled the carcass of gorilla, chimpanzee, or duiker.[18] Fruit bats are also reported to be a treat eaten by people in parts of West Africa where they are smoked, grilled or made into a spicy soup.[15] The virus then spreads person-to-person, especially within families, hospitals, and during some mortuary rituals where contact among individuals becomes more likely.[19]
    The virus has been confirmed to be transmitted through body fluids. Transmission through oral exposure and through conjunctiva exposure is likely[20] and has been confirmed in non-human primates.[21] Filoviruses are not naturally transmitted by aerosol. They are, however, highly infectious as breathable 0.8–1.2 micrometre droplets in laboratory conditions;[22] because of this potential route of infection, these viruses have been classified as Category A biological weapons.[23]
    All epidemics of Ebola have occurred in sub-optimal hospital conditions, where practices of basic hygiene and sanitation are often either luxuries or unknown to caretakers and where disposable needles and autoclaves are unavailable or too expensive. In modern hospitals with disposable needles and knowledge of basic hygiene and barrier nursing techniques, Ebola has never spread on a large scale. In isolated settings such as a quarantined hospital or a remote village, most victims are infected shortly after the first case of infection is present. The quick onset of symptoms from the time the disease becomes contagious in an individual makes it easy to identify sick individuals and limits an individual's ability to spread the disease by traveling. Because bodies of the deceased are still infectious, some doctors had to take measures to properly dispose of dead bodies in a safe manner despite local traditional burial rituals.[24]

    Virology

    Main article: Ebola virus
    Genus Ebolavirus: species and their EVD-causing viruses
    Species name Virus name (Abbreviation)
    Bundibugyo ebolavirus (accepted)[25] Bundibugyo virus (BDBV; previously BEBOV)
    Sudan ebolavirus Sudan virus (SUDV; previously SEBOV)
    Taï Forest ebolavirus Taï Forest virus (TAFV; previously CIEBOV)
    Zaire ebolavirus* Ebola virus (EBOV; previously ZEBOV)
    Table legend: "*" denotes the type species and "accepted" refers to a taxon that has been accepted by the Executive Committee of the ICTV but that has yet to be ratified.

    Genome


    Electron micrograph of an Ebola virus virion
    Like all mononegaviruses, ebolavirions contain linear nonsegmented, single-strand, non-infectious RNA genomes of negative polarity that possesses inverse-complementary 3' and 5' termini, do not possess a 5' cap, are not polyadenylated, and are not covalently linked to a protein.[26] Ebolavirus genomes are approximately 19 kilobase pairs long and contain seven genes in the order 3'-UTR-NP-VP35-VP40-GP-VP30-VP24-L-5'-UTR.[27] The genomes of the five different ebolaviruses (BDBV, EBOV, RESTV, SUDV, and TAFV) differ in sequence and the number and location of gene overlaps.

    Structure

    Like all filoviruses, ebolavirions are filamentous particles that may appear in the shape of a shepherd's crook or in the shape of a "U" or a "6", and they may be coiled, toroid, or branched.[27] In general, Ebolavirions are 80 nm in width, but vary somewhat in length. In general, the median particle length of ebolaviruses ranges from 974 to 1,086 nm (in contrast to marburgvirions, whose median particle length was measured to be 795–828 nm), but particles as long as 14,000 nm have been detected in tissue culture.[28] Ebolavirions consist of seven structural proteins. At the center is the helical ribonucleocapsid, which consists of the genomic RNA wrapped around a polymer of nucleoproteins (NP). Associated with the ribonucleoprotein is the RNA-dependent RNA polymerase (L) with the polymerase cofactor (VP35) and a transcription activator (VP30). The ribonucleoprotein is embedded in a matrix, formed by the major (VP40) and minor (VP24) matrix proteins. These particles are surrounded by a lipid membrane derived from the host cell membrane. The membrane anchors a glycoprotein (GP1,2) that projects 7 to 10 nm spikes away from its surface. While nearly identical to marburgvirions in structure, ebolavirions are antigenically distinct.

    Entry

    Niemann–Pick C1 (NPC1) appears to be essential for Ebola infection. Two independent studies reported in the same issue of Nature showed that Ebola virus cell entry and replication requires the cholesterol transporter protein NPC1.[29][30] When cells from Niemann-Pick disease, type C1 patients (who have a mutated form of NPC1) were exposed to Ebola virus in the laboratory, the cells survived and appeared immune to the virus, further indicating that Ebola relies on NPC1 to enter cells. This might imply that genetic mutations in the NPC1 gene in humans could make some people resistant to one of the deadliest known viruses affecting humans. The same studies described similar results with Ebola's cousin in the filovirus group, Marburg virus, showing that it too needs NPC1 to enter cells.[29][30] Furthermore, NPC1 was shown to be critical to filovirus entry because it mediates infection by binding directly to the viral envelope glycoprotein.[30] A later study confirmed the findings that NPC1 is a critical filovirus receptor that mediates infection by binding directly to the viral envelope glycoprotein and that the second lysosomal domain of NPC1 mediates this binding.[31]
    In one of the original studies, a small molecule was shown to inhibit Ebola virus infection by preventing the virus glycoprotein from binding to NPC1.[30][32] In the other study, mice that were heterozygous for NPC1 were shown to be protected from lethal challenge with mouse adapted Ebola virus.[29] Together, these studies suggest NPC1 may be potential therapeutic target for an Ebola anti-viral drug.

    Replication

    The ebolavirus life cycle begins with virion attachment to specific cell-surface receptors, followed by fusion of the virion envelope with cellular membranes and the concomitant release of the virus nucleocapsid into the cytosol. The viral RNA polymerase, encoded by the L gene, partially uncoats the nucleocapsid and transcribes the genes into positive-strand mRNAs, which are then translated into structural and nonstructural proteins. Ebolavirus RNA polymerase (L) binds to a single promoter located at the 3' end of the genome. Transcription either terminates after a gene or continues to the next gene downstream. This means that genes close to the 3' end of the genome are transcribed in the greatest abundance, whereas those toward the 5' end are least likely to be transcribed. The gene order is, therefore, a simple but effective form of transcriptional regulation. The most abundant protein produced is the nucleoprotein, whose concentration in the cell determines when L switches from gene transcription to genome replication. Replication results in full-length, positive-strand antigenomes that are, in turn, transcribed into negative-strand virus progeny genome copy. Newly synthesized structural proteins and genomes self-assemble and accumulate near the inside of the cell membrane. Virions bud off from the cell, gaining their envelopes from the cellular membrane they bud from. The mature progeny particles then infect other cells to repeat the cycle.[33]

    Pathophysiology


    Pathogenesis schematic
    Endothelial cells, mononuclear phagocytes, and hepatocytes are the main targets of infection. After infection, a secreted glycoprotein (sGP) known as the Ebola virus glycoprotein (GP) is synthesized. Ebola replication overwhelms protein synthesis of infected cells and host immune defenses. The GP forms a trimeric complex, which binds the virus to the endothelial cells lining the interior surface of blood vessels. The sGP forms a dimeric protein that interferes with the signaling of neutrophils, a type of white blood cell, which allows the virus to evade the immune system by inhibiting early steps of neutrophil activation. These white blood cells also serve as carriers to transport the virus throughout the entire body to places such as the lymph nodes, liver, lungs, and spleen.[34] The presence of viral particles and cell damage resulting from budding causes the release of cytokines (to be specific, TNF-α, IL-6, IL-8, etc.), which are the signaling molecules for fever and inflammation. The cytopathic effect, from infection in the endothelial cells, results in a loss of vascular integrity. This loss in vascular integrity is furthered with synthesis of GP, which reduces specific integrins responsible for cell adhesion to the inter-cellular structure, and damage to the liver, which leads to coagulopathy.[35]

    Diagnosis

    EVD cannot be separated from Marburg virus disease based on symptoms. It can also easily be confused with many other diseases common in Equatorial Africa such as other viral hemorrhagic fevers, falciparum malaria, typhoid fever, shigellosis, rickettsial diseases such as typhus, cholera, gram-negative septicemia, borreliosis such as relapsing fever or EHEC enteritis. Other infectious diseases that should be included in the differential diagnosis include the following: leptospirosis, scrub typhus, plague, Q fever, candidiasis, histoplasmosis, trypanosomiasis, visceral leishmaniasis, hemorrhagic smallpox, measles, and fulminant viral hepatitis.[citation needed] Non-infectious diseases that can be confused with EVD are acute promyelocytic leukemia, hemolytic uremic syndrome, snake envenomation, clotting factor deficiencies/platelet disorders, thrombotic thrombocytopenic purpura, hereditary hemorrhagic telangiectasia, Kawasaki disease, and even warfarin intoxication.[36][37][38][39]
    EVD's most important clinical indicator is the persons medical history, especially travel and occupational history and the patient's exposure to wildlife. EVD can be confirmed by isolating ebolaviruses from or by detection of ebolavirus antigen or genomic or subgenomic RNAs in patient blood or serum samples during the acute phase of EVD. Ebolavirus isolation is usually performed by inoculation of grivet kidney epithelial Vero E6 or MA-104 cell cultures or by inoculation of human adrenal carcinoma SW-13 cells, all of which reacting to infection with characteristic cytopathic effects.[40][41] Filovirions can easily be visualized and identified in cell culture by electron microscopy due to their unique filamentous shapes, but electron microscopy cannot differentiate the various filoviruses alone despite some overall length differences.[28] Immunofluorescence assays are used to confirm ebolavirus presence in cell cultures. During an outbreak, virus isolation and electron microscopy are most often not feasible options. The most common diagnostic methods are therefore RT-PCR[42][43][44][45][46][47][48] in conjunction with antigen-capture ELISA[49][50][51][52][53] which can be performed in field or mobile hospitals and laboratories. Indirect immunofluorescence assays (IFAs) are not used for diagnosis of EVD in the field anymore.

    Classification

    The genera Ebolavirus and Marburgvirus were originally classified as the species of the now-obsolete Filovirus genus. In March 1998, the Vertebrate Virus Subcommittee proposed in the International Committee on Taxonomy of Viruses (ICTV) to change the Filovirus genus to the Filoviridae family with two specific genera: Ebola-like viruses and Marburg-like viruses. This proposal was implemented in Washington, DC on April 2001 and in Paris on July 2002. In 2000, another proposal was made in Washington, D.C., to change the "-like viruses" to "-virus" resulting in today's Ebolavirus and Marburgvirus.[54]

    Phylogenetic tree comparing the Ebolavirus and Marburgvirus. Numbers indicate percent confidence of branches.
    Rates of genetic change are 100 times slower than influenza A in humans, but on the same magnitude as those of hepatitis B. Extrapolating backwards using these rates indicates that Ebolavirus and Marburgvirus diverged several thousand years ago.[55] However, paleoviruses (genomic fossils) of filoviruses (Filoviridae) found in mammals indicate that the family itself is at least tens of millions of years old.[16] Fossilized viruses that are closely related to ebolaviruses have been found in the genome of the Chinese hamster.[56]
    The five characterised Ebola species are:
    Zaire ebolavirus (EBOV; previously ZEBOV) 
    Also known simply as the Zaire virus, ZEBOV has the highest case-fatality rate of the ebolaviruses, up to 90% in some epidemics, with an average case fatality rate of approximately 83% over 27 years. There have been more outbreaks of Zaire ebolavirus than of any other species. The first outbreak occurred on 26 August 1976 in Yambuku.[57] The first recorded case was Mabalo Lokela, a 44‑year-old schoolteacher. The symptoms resembled malaria, and subsequent patients received quinine. Transmission has been attributed to reuse of unsterilized needles and close personal contact.
    Sudan ebolavirus (SUDV; previously SEBOV) 
    Like the Zaire virus, SEBOV emerged in 1976; it was at first assumed to be identical with the Zaire species.[58] SEBOV is believed to have broken out first among cotton factory workers in Nzara, Sudan (now South Sudan), with the first case reported as a worker exposed to a potential natural reservoir. The virus was not found in any of the local animals and insects that were tested in response. The carrier is still unknown. The lack of barrier nursing (or "bedside isolation") facilitated the spread of the disease. The most recent outbreak occurred in May, 2004. Twenty confirmed cases were reported in Yambio County, Sudan (now South Sudan), with five deaths resulting. The average fatality rates for SEBOV were 54% in 1976, 68% in 1979, and 53% in 2000 and 2001.
    Reston ebolavirus (RESTV; previously REBOV) 
    Discovered during an outbreak of simian hemorrhagic fever virus (SHFV) in crab-eating macaques from Hazleton Laboratories (now Covance) in 1989. Since the initial outbreak in Reston, Virginia, it has since been found in non-human primates in Pennsylvania, Texas and Siena, Italy. In each case, the affected animals had been imported from a facility in the Philippines,[59] where the virus has also infected pigs.[60] Despite having a Biosafety status of Level‑4 and its apparent pathogenicity in monkeys, REBOV did not cause disease in exposed human laboratory workers.[61]
    Côte d'Ivoire ebolavirus (TAFV; previously CIEBOV)
    Also referred to as Taï Forest ebolavirus and by the English place name, "Ivory Coast", it was first discovered among chimpanzees from the Taï Forest in Côte d'Ivoire, Africa, in 1994. Necropsies showed blood within the heart to be brown; no obvious marks were seen on the organs; and one necropsy showed lungs filled with blood. Studies of tissues taken from the chimpanzees showed results similar to human cases during the 1976 Ebola outbreaks in Zaire and Sudan. As more dead chimpanzees were discovered, many tested positive for Ebola using molecular techniques. The source of the virus was believed to be the meat of infected Western Red Colobus monkeys, upon which the chimpanzees preyed. One of the scientists performing the necropsies on the infected chimpanzees contracted Ebola. She developed symptoms similar to those of dengue fever approximately a week after the necropsy, and was transported to Switzerland for treatment. She was discharged from the hospital after two weeks and had fully recovered six weeks after the infection.[62]
    Bundibugyo ebolavirus (BDBV; previously BEBOV)
    On 24 November 2007, the Uganda Ministry of Health confirmed an outbreak of Ebolavirus in the Bundibugyo District. After confirmation of samples tested by the United States National Reference Laboratories and the CDC, the World Health Organization confirmed the presence of the new species. On 20 February 2008, the Uganda Ministry officially announced the end of the epidemic in Bundibugyo, with the last infected person discharged on 8 January 2008.[63] An epidemiological study conducted by WHO and Uganda Ministry of Health scientists determined there were 116 confirmed and probable cases of the new Ebola species, and that the outbreak had a mortality rate of 34% (39 deaths). In 2012, there was an outbreak of Bundibugyo ebolavirus in a northeastern province of the Democratic Republic of the Congo. There were 15 confirmed cases and 10 fatalities.[64]

    Prevention


    A researcher working with the Ebola virus while wearing a BSL-4 positive pressure suit to avoid infection
    Ebola viruses are highly infectious as well as contagious. Governments and individuals often quickly respond to quarantine the area while the lack of roads and transportation in many parts of Africa helps to contain the outbreak.[59] Airline crews are trained to spot the symptoms of Ebola in passengers flying from places where the virus is found. Crews are told to quarantine anyone who looks infected. [65]
    As an outbreak of ebola progresses, bodily fluids from diarrhea, vomiting, and bleeding represent a hazard. Due to lack of proper equipment and hygienic practices, large-scale epidemics occur mostly in poor, isolated areas without modern hospitals or well-educated medical staff. Many areas where the infectious reservoir exists have just these characteristics. In such environments, all that can be done is to immediately cease all needle-sharing or use without adequate sterilization procedures, isolate patients, and observe strict barrier nursing procedures with the use of a medical-rated disposable face mask, gloves, goggles, and a gown at all times, strictly enforced for all medical personnel and visitors.[66] The aim of all of these techniques is to avoid any person’s contact with the blood or secretions of any patient, including those who are deceased.[67]
    Vaccines have protected nonhuman primates. The six months needed for immunization impede counter-epidemic uses. In 2003, a vaccine using an adenoviral (ADV) vector carrying the Ebola spike protein therefore was tested on crab-eating macaques. The monkeys twenty-eight days later were challenged with the virus and remained resistant.[68] A vaccine based on attenuated recombinant vesicular stomatitis virus (VSV) vector carrying either the Ebola glycoprotein or the Marburg glycoprotein in 2005 protected nonhuman primates,[69] opening clinical trials in humans.[70] The study by October completed the first human trial, over three months giving three vaccinations safely inducing an immune response. Individuals for a year were followed, and, in 2006, a study testing a faster-acting, single-shot vaccine began; this new study was completed in 2008.[71] Trying the vaccine on a strain of Ebola that more resembles the one that infects humans is the next step.[citation needed]
    The Food and Drug Administration has approved no candidate vaccines,[72][73][74] the most promising whereof are DNA vaccines[75] or derive from adenoviruses,[68] vesicular stomatitis Indiana virus (VSIV)[76][77][78] or filovirus-like particles (VLPs)[79] because these candidates could protect nonhuman primates from ebolavirus-induced disease. DNA vaccines, adenovirus-based vaccines, and VSIV-based vaccines have entered clinical trials.[70][71][80][81]
    Ebolaviruses are not transmitted by aerosol during natural EVD outbreaks. Without an approved vaccine, EVD prevention predominantly involves behavior modification, proper personal protective equipment, and sterilization/disinfection.
    On 6 December 2011, the development of a successful vaccine against Ebola for mice was reported. Unlike the predecessors, it can be freeze-dried and thus stored for long periods in wait for an outbreak. The research is reported in Proceedings of National Academy of Sciences.[82]

    Endemic zones

    The natural maintenance hosts of ebolaviruses are unidentified: primary infection cannot necessarily be prevented in nature. Avoiding EVD such risk factors as contact with bats or nonhuman primates therefore is highly recommended and may be impossible for inhabitants of tropical forests or people dependent on nonhuman primates as a food source.

    During outbreaks

    The most straightforward prevention method during EVD outbreaks is not touching patients, their excretions, and body fluids, or possibly contaminated materials and utensils. Patients should be isolated, and medical staff should be trained and apply strict barrier nursing techniques (disposable face mask, gloves, goggles, and a gown at all times). Traditional burial rituals, especially those requiring embalming of bodies, should be discouraged or modified.[66]

    In the laboratory

    Ebola viruses are World Health Organization Risk Group 4 Pathogens, requiring Biosafety Level 4-equivalent containment. Laboratory researchers have to be properly trained in BSL-4 practices and wear proper personal protective equipment.

    Treatment


    A hospital isolation ward in Gulu, Uganda, during the October 2000 outbreak
    No ebolavirus-specific treatment exists. Treatment is primarily supportive in nature and includes minimizing invasive procedures, balancing fluids and electrolytes to counter dehydration, administration of anticoagulants early in infection to prevent or control disseminated intravascular coagulation, administration of procoagulants late in infection to control hemorrhaging, maintaining oxygen levels, pain management, and administration of antibiotics or antimycotics to treat secondary infections.[83][84][85]

    Experimental therapies

    Hyperimmune equine immunoglobulin raised against EBOV has been used in Russia to treat a laboratory worker who accidentally infected herself with EBOV—but the patient died anyway.[86] Experimentally, recombinant vesicular stomatitis Indiana virus (VSIV) expressing the glycoprotein of EBOV or SUDV has been used successfully in nonhuman primate models as post-exposure prophylaxis.[87][88] Such a recombinant post-exposure vaccine was also used to treat a German researcher who accidentally pricked herself with a possibly EBOV-contaminated needle. Treatment might have been successful as she survived. However, actual EBOV infection could never be demonstrated without a doubt.[89] Novel, very promising, experimental therapeutic regimens rely on antisense technology. Both small interfering RNAs (siRNAs) and phosphorodiamidate morpholino oligomers (PMOs) targeting the EBOV genome could prevent disease in nonhuman primates.[90][91]
    Researchers from the U.S. Army Medical Research Institute of Infectious Diseases also found that FDA-Approved estrogen receptor drugs used to treat infertility and breast cancer (clomiphene and toremifene) inhibit the progress of Ebola virus in infected mice.[92] Ninety percent of the mice treated with clomiphene and 50 percent of those treated with toremifene survived the tests.[93] The authors of the study concluded that given their oral availability and history of human use, these drugs would be excellent candidates for repurposing efforts to treat Ebola virus infection in remote geographical locations, either on their own or together with other antiviral drugs.
    During an outbreak in the Democratic Republic of the Congo in 1995, seven of eight patients having received blood transfusions from convalescent individuals survived.[94] However, this potential treatment is considered controversial.[58]

    Prognosis

    In general, outcomes are poor with 68% of all cases resulting in death[citation needed]. If an infected person survives, recovery may be quick and complete, or prolonged with long term problems, such as inflammation of the testicles, joint pains, muscle pains, skin peeling, or hair loss. Eye symptoms, such as light sensitivity, excess tearing, iritis, iridocyclitis, choroiditis and blindness have also been described. EBOV and SUDV may be able to persist in the sperm of some survivors, which could give rise to secondary infections and disease via sexual intercourse.[1]

    Epidemiology

    For more about specific outbreaks and their descriptions, see List of Ebola outbreaks.
    Outbreaks of EVD have occurred mainly in Africa.
    Ebola virus disease (EVD) outbreaks
    Year Virus Geographic Location Human Deaths Cases CFR (case-fatality risk)
    1976 SUDV Juba, Maridi, Nzara, and Tembura, Sudan 151 284 53%
    1976 EBOV Yambuku, Zaire 280 318 88%
    1977 EBOV Bonduni, Zaire 1 1 100%
    1979 SUDV Nzara, Sudan 22 34 65%
    1988 EBOV Porton Down, United Kingdom [laboratory accident] 0 1 0%
    1994 TAFV Taï National Park, Côte d'Ivoire 0 1 0%
    1994–1995 EBOV Woleu-Ntem and Ogooué-Ivindo Provinces, Gabon 32 52 62%
    1995 EBOV Kikwit, Zaire 245 317 77%
    1996 EBOV Mayibout 2, Gabon 21 31 68%
    1996 EBOV Sergiyev Posad, Russia [laboratory accident] 1 1 100%
    1996–1997 EBOV Ogooué-Ivindo Province, Gabon; Cuvette-Ouest Department, Republic of the Congo 46 62 74%
    2000–2001 SUDV Gulu, Mbarara, and Masindi Districts, Uganda 224 425 53%
    2001–2002 EBOV Ogooué-Ivindo Province, Gabon; Cuvette-Ouest Department, Republic of the Congo 97 124 78%
    2002 EBOV Ogooué-Ivindo Province, Gabon; Cuvette-Ouest Department, Republic of the Congo 10 11 91%
    2002–2003 EBOV Cuvette-Ouest Department, Republic of the Congo; Ogooué-Ivindo Province, Gabon 128 143 90%
    2003–2004 EBOV Cuvette-Ouest Department, Republic of the Congo 29 35 83%
    2004 EBOV Koltsovo, Russia [laboratory accident] 1 1 100%
    2004 SUDV Yambio County, Sudan 7 17 41%
    2005 EBOV Cuvette-Ouest Department, Republic of the Congo 9 11 82%
    2007 EBOV Kasai Occidental Province, Democratic Republic of the Congo 186 264 71%
    2007–2008 BDBV Bundibugyo District, Uganda 39 116 34%
    2008–2009 EBOV Kasai Occidental Province, Democratic Republic of the Congo 15 32 47%
    2011 SUDV Luweero District, Uganda 1 1 100%
    2012 SUDV Kibaale District, Western Uganda 17 24 71%
    2012 BDBV Orientale Province, Democratic Republic of the Congo 34 62 54%
    2014 EBOV Guinea, Sierra Leone, Liberia [95] 337 528 (364 laboratory confirmed) 64%

    CDC worker incinerates med-waste from Ebola patients in Zaire in 1976
    While investigating an outbreak of Simian hemorrhagic fever virus (SHFV) in November 1989, an electron microscopist from USAMRIID discovered filoviruses similar in appearance to Ebola in tissue samples taken from crab-eating macaque imported from the Philippines to Hazleton Laboratories Reston, Virginia.[96]
    Blood samples were taken from 178 animal handlers during the incident.[97] Of those, six animal handlers eventually seroconverted. When the handlers failed to become ill, the CDC concluded that the virus had a very low pathogenicity to humans.[98]
    Because of the virus's high mortality, it is a potential agent for biological warfare.[99]
    Given the lethal nature of Ebola, and since no approved vaccine or treatment is available, it is classified as a biosafety level 4 agent, as well as a Category A bioterrorism agent by the Centers for Disease Control and Prevention. It has the potential to be weaponized for use in biological warfare.[100] The BBC reports in a study that frequent outbreaks of Ebola may have resulted in the deaths of 5,000 gorillas.[101]

    2007 to 2011

    As of 30 August 2007, 103 people (100 adults and three children) were infected by a suspected hemorrhagic fever outbreak in the village of Kampungu, Democratic Republic of the Congo. The outbreak started after the funerals of two village chiefs, and 217 people in four villages fell ill. The World Health Organization sent a team to take blood samples for analysis and confirmed that many of the cases are the result of Ebolavirus.[102][103] The Congo's last major Ebola epidemic killed 245 people in 1995 in Kikwit, about 200 miles (320 km) from the source of the August 2007 outbreak.[104]
    On 30 November 2007, the Uganda Ministry of Health confirmed an outbreak of Ebola in the Bundibugyo District. After confirmation of samples tested by the United States National Reference Laboratories and the Centers for Disease Control, the World Health Organization confirmed the presence of a new species of Ebolavirus, which is now tentatively named Bundibugyo.[105] The epidemic came to an official end on 20 February 2008. While it lasted, 149 cases of this new strain were reported, and 37 of those led to deaths.
    An International Symposium to explore the environment and filovirus, cell system and filovirus interaction, and filovirus treatment and prevention was held at Centre Culturel Français, Libreville, Gabon, during March 2008.[106] The virus appeared in southern Kasai Occidental on 27 November 2008,[107] and blood and stool samples were sent to laboratories in Gabon and South Africa for identification.
    On 25 December 2008, a mysterious disease that had killed 11 and infected 21 people in southern Democratic Republic of Congo was identified as the Ebola virus.[108] Doctors Without Borders reported 11 deaths as of 29 December 2008 in the Western Kasai province of the Democratic Republic of Congo, stating that a further 24 cases were being treated. In January 2009, Angola closed down part of its border with DRC to prevent the spread of the outbreak.[109]
    On 12 March 2009, an unidentified 45-year-old scientist from Germany accidentally pricked her finger with a needle used to inject Ebola into lab mice. She was given an experimental vaccine never before used on humans. Since the peak period for an outbreak during the 21-day Ebola incubation period has passed as of 2 April 2009, she has been declared healthy and safe. It remains unclear whether or not she was ever actually infected with the virus.[110]
    In May 2011, a 12-year-old girl in Uganda died from Ebola (Sudan subspecies). No further cases were recorded.[111]

    2012 outbreaks

    In July 2012, the Ugandan Health Ministry confirmed 13 deaths due to an outbreak of the Ebola-Sudan variant[112] in the Kibaale District.[113] As of 28 July 2012, 14 out of 20 (70% mortality rate) had died in Kibaale.[114] On July 30, Stephen Byaruhanga, a health official in Kibaale District, said the Ebola outbreak has spread from one remote village to several villages.[115]
    The World Health Organization's global and alert response network reported on August 3 that the suspected case count had risen to 53, including 16 deaths. Of these cases, five were confirmed by UVRI as Ebola cases. There have been no confirmed cases outside of Kibaale District except for a patient who was medically evacuated to Kampala District and has since died. WHO and CDC support is on the ground in Uganda supporting the government response. There have been no confirmed cases outside of Uganda.[116] Included among the populations confirmed to be affected are prisoners in Kabbale prison. One of the inmates suspected of infection escaped from medical isolation on the same day.[117] Dr. Joaquim Saweka, the WHO representative to Uganda, also reported that the outbreak was then under control and that everyone known to have had contact with a known Ebola patient is now in isolation.[118]
    On 8 August 2012, the Ugandan Ministry of Health has recorded 23 probable and confirmed cases, including 16 deaths. Ten cases were confirmed by the Uganda Virus Research Institute as Ebola. 185 people who came into contact with probable and confirmed Ebola cases are being followed up during the incubation period of 21 days.[119]
    On 17 August 2012, the Ministry of Health of the Democratic Republic of the Congo reported an outbreak of the Ebola-Bundibugyo variant[120] in the eastern region.[121] By August 21, the WHO reported a total of 15 cases and 10 fatalities.[122] No evidence suggests that this outbreak connects to the Ugandan outbreak.[123] By 13 September 2012, the World Health Organisation revealed that the virus had claimed 32 lives and that the probable cause of the outbreak was tainted bush-meat hunted by local villagers around the towns of Isiro and Viadana.[124]

    2014 outbreak

    In February 2014, a strain of the Ebola Virus appeared in Guinea. This is the first Ebola virus outbreak registered in the region. As of April 10, 157 suspected and confirmed cases and 101 deaths have been reported in Guinea, 22 suspected cases in Liberia including 14 deaths, 8 suspected cases in Sierra Leone including 6 deaths, and 1 suspected case in Mali.[125][126] Investigations on these are under way.[127][128][129]

    History

    For more about the outbreak in Virginia, see Reston virus.

    Cases of ebola fever in Africa from 1979 to 2008.
    Ebola virus first emerged in 1976 in outbreaks of Ebola hemorrhagic fever in Zaire and Sudan.[130] The strain of Ebola that broke out in Zaire has one of the highest case fatality rates of any human virus, roughly 90%.[131]
    The name of the disease originated from one of those first recorded outbreaks in 1976 in Yambuku, Democratic Republic of the Congo (then Zaire) which lies on the Ebola River.
    The Philippines and the United States had no previous cases of infection, and upon further isolation it was concluded to be another strain of Ebola or a new filovirus of Asian origin, and named Reston ebolavirus (REBOV) after the location of the incident.
    Some scientists also believe that the Plague of Athens, which wiped out about a third of its inhabitants during the Peloponnesian War, may have been caused by Ebola. However, these studies are conflicting, and point to other possible diseases such as typhoid.[132]

    Other animals

    In general, outbreaks of EVD among human populations result from handling infected wild animal carcasses. In general, declines in animal populations precede outbreaks among human populations. Since 2003, such declines have been monitored through surveillance of animal populations with the aim of predicting and preventing EVD outbreaks in humans.[133] Recovered carcasses from gorillas contain multiple Ebola virus strains, which suggest multiple introductions of the virus. Bodies decompose quickly and carcasses are not infectious after three to four days. Contact between gorilla groups is rare, suggesting transmission among gorilla groups is unlikely, and that outbreaks result from transmission between viral reservoir and animal populations.[134]
    Outbreaks of EVD may have been responsible for an 88% decline in tracking indices of observed chimpanzee populations in 420 square kilometer Lossi Sanctuary between 2002 and 2003.[134] Transmission among chimpanzees through meat consumption constitutes a significant 5.2 (1.3–21.1 with 95% confidence) relative risk factor, while contact between individuals, such as touching dead bodies and grooming, do not.[135]

    Domestic animals

    Ebola virus can be transmitted to dogs and pigs.[136] While dogs may be asymptomatic, pigs tend to develop clinical disease.

    Recent research

    In late 2012, Canadian scientists discovered that the deadliest form of the virus could be transmitted by air between species.[137] They managed to prove that the virus was transmitted from pigs to monkeys without any direct contact between them, leading to fears that airborne transmission could be contributing to the wider spread of the disease in parts of Africa. Evidence was also found that pigs might be one of the reservoir hosts for the virus; the fruit bat has long been considered as the reservoir.[137]

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